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Chemistry 23-086

Scalable fabrication of self-assembled cell sheet

Tech ID



P. Ravi Selvagnapathy
Maedeh khodamoradi

Patent Status

US provisional filed

Stage of Research

Development stage


Tao Li
Business Development Officer



Cell sheet tissue engineering is a scaffold-free technique that allows the fabrication of in vivo-like tissues, especially cell-dense and transparent tissues such as the heart and cornea. However, existing methods for fabricating cell sheets show some limitations such as a long culture period, extensive substrate treatment, fragility, and relatively small size of obtained cell sheets. Researchers at McMaster University have developed a simple and scalable fabrication method for self-assembled cell sheets, which utilizes a low surface energy and ultralow adhesion substrate to culture cells and form a self-assembled and scaffold-free cell sheet within a short incubation time. They have also developed methods to assemble them into patterns and large area constructs.


  • Cell rich tissue regeneration (heart and skeletal muscle tissue engineering)
  • Cultivating meat production by using cell sheets made with skeletal muscle cells and adipocytes
  • Making skin for grafts by stacking cell sheets made with fibroblasts, keratinocytes, and stem cells
  • Ocular tissue engineering and in vitro disease modeling (cornea and retina tissues)


  • Rapid formation of a cell sheet (4-6hrs incubation)
  • Ultra-low adhesion between cell sheet and substrate
  • Customizable pattern and coculture for cell sheet
  • Customizable shape and thickness of cell sheet
  • Easy construction of complex structure with cell sheets
  • Inexpensive procedure

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