Abstract

Ion exchange membrane chromatography is widely used for purification of proteins and other biomacromolecules. However, existing methods are not suitable for high-resolution purification. To address this issue, researchers at McMaster University have developed a modulated membrane chromatography device that combines a positively charged binding membrane and a negatively charged modulating membrane, which can separate biological macromolecules with high-resolution.

Applications

Separation of biological macromolecules, including carbohydrates such as dextran, lipids such as fatty acids, proteins such as enzymes and antibodies, and nucleic acids such as RNA and DNA

Advantages

• High-resolution of separation
• Low-cost of manufacture
• Purification of hard to separate proteins such as aggregates and variants